Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2788062 | Journal of Genetics and Genomics | 2008 | 7 Pages |
Abstract
Î12 fatty acid desaturase gene has been targeted as a logical candidate controlling the high oleate trait in peanut seeds. By RT-PCR method, the full-length cDNAs of Î12 fatty acid desaturase gene were isolated from peanut (Arachis hypogaea L.) genotypes with normal and high ratio of oleic to linoleic acid, which were designated AhFAD2B and AhFAD2Bâ², respectively. Sequence alignment of their coding regions revealed that an extra A was inserted at the position +442 bp of AhFAD2Bâ² sequence of high oleic acid genotypes, which resulted in the shift of open reading frame and a truncated protein AhFAD2Bâ², with the loss of one histidine box involved in metal ion complex required for the reduction of oxygen. Analysis of transcript level showed that the expression of Î12 fatty acid desaturase gene in high oleic acid genotype was slightly lower than that in normal genotype. The enzyme activity experiment of yeast (Saccharomyces cerevisiae) cell transformed with AhFAD2B or AhFAD2Bâ² proved that only AhFAD2B gene product showed significant Î12 fatty acid desaturase activity, but AhFAD2Bâ² gene product did not. These results suggested that the change of AhFAD2Bâ² gene sequence resulted in lower activity or deactivation of Î12 fatty acid desaturase in high oleic acid genotype.
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Authors
Shanlin Yu, Lijuan Pan, Qingli Yang, Ping Min, Zengkai Ren, Hongsheng Zhang,