Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2795359 | Cytokine | 2010 | 4 Pages |
Abstract
C-reactive protein (CRP) has been demonstrated to induce blood–brain barrier disruption (BBB) involving NAD(P)H-oxidase dependent oxidative stress. It is unclear why CRP affects the BBB and not other vascular beds following stroke. Therefore we examined CRP receptor and NAD(P)H-oxidase expression levels in bovine brain- (BEC) and aortic endothelial cells. Dichlorodihydrofluorescein measurements revealed significantly higher CRP-induced reactive oxygen species (ROS) levels in BEC. Protein expression of the CRP-receptors CD16, CD32 and of the NAD(P)H-oxidase subunit p22phox were also significantly higher in BEC. In conclusion BEC show a higher vulnerability to CRP due to increased levels of CRP receptors and the NAD(P)H-oxidase.
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Authors
Dorothea Closhen, Bianca Bender, Heiko J. Luhmann, Christoph R.W. Kuhlmann,