Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2798001 | Diabetes Research and Clinical Practice | 2008 | 6 Pages |
We investigated the effects of fasting on gene expression and intracellular signals regulating energy metabolism in adipose tissue. Following fasting for 15 h or 39 h, epididymal fat pads were isolated from Wistar rats. PPARγ mRNA levels decreased in the adipose tissues isolated from rats fasted for 39 h, whereas adipocyte lipid-binding protein (aP2) and lipoprotein lipase (LPL) mRNA levels increased. Overnight fasting increased the AMP/ATP ratio and AMP-activated protein kinase (AMPK) in adipose tissue, but not in muscle or liver tissue. In addition, the effect of 5-aminoimidazole-4-carboxyamide-ribonucleoside (AICAR) on PPARγ expression in primary cultured adipocytes was investigated. AICAR reduced PPARγ mRNA levels but increased aP2 and LPL mRNA levels. Thus, fasting-induced AMPK activation may affect on the regulation of gene expression in adipocytes.