Dual role of IGF-II in oocyte maturation in southern flounder Paralichthys lethostigma: Up-regulation of mPRα and resumption of meiosis

Increasing evidence suggests a regulatory role for the IGF system in teleost oocyte maturation (OM). Our objectives were to determine if IGF-I and IGF-II regulate different stages of OM in southern flounder (Paralichthys lethostigma) and to identify the likely maturation-inducing steroid (MIS) in this species. The most abundant final product of ovarian steroidogenesis assays eluted at the position of 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S). 20β-S was also more potent in inducing germinal vesicle breakdown (GVBD) of maturationally-competent oocytes than other teleost MISs. IGF-II (100 nM) induced maturational competence (OMC), as greater GVBD was induced after incubation with IGF-II + 20β-S compared to that of the 20β-S + 20β-S or IGF-II + no treatment group. Incubation with IGF-II (100 nM) for 4–8 h significantly increased ovarian membrane progestin receptor alpha (mPRα or Paqr7b) mRNA levels 12–15% and mPRα protein levels 75–101%. Further, the IGF-II-induced increase in mPRα protein concentrations was partially blocked by pretreatment with Wortmannin, a Pik3 inhibitor, and PD 098,059, a Mapk inhibitor. Both IGF-I and -II (100 nM) induced GVBD of maturationally-competent oocytes was blocked by incubation with cycloheximide. Incubation with d,l-Aminoglutethimide decreased IGF-II-induced GVBD but had no effect on IGF-I-induced GVBD. IGF-I and -II were also able to induce GVBD of maturationally-incompetent oocytes, and elicited 75% and 135% greater GVBD, respectively, than hCG + 20β-S at 100 nM. In conclusion, we show that 20β-S is the likely MIS in this species and that IGF-I and -II are also able to induce GVBD. Further, IGF-II not only induces OMC but also up-regulates ovarian mPRα mRNA and protein through Pik3- and Mapk-dependent pathways. This is the first demonstration of mPRα regulation by an IGF in any vertebrate species.

► IGF-II up-regulates membrane progestin receptor alpha during oocyte maturation in fish. ► IGF-I and -II induce maturational competence and meiosis of flounder oocytes. ► IGFs regulate both stages of oocyte maturation in southern flounder. ► 20β-S is the likely maturation-inducing steroid in southern flounder.