Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2802240 | General and Comparative Endocrinology | 2006 | 8 Pages |
Abstract
Secretion of α-melanophore-stimulating hormone (αMSH) from pituitary melanotrope cells of the amphibian Xenopus laevis is under inhibitory synaptic control by three neurotransmitters produced by the suprachiasmatic nucleus: γ-aminobutyric acid (GABA), neuropeptide Y (NPY) and dopamine (DA). These inhibitory effects occur through Gi-protein-coupled receptors (GiPCR), and differ in strength: GABAB-receptor-induced inhibition is the weakest, whereas DA (via a D2-receptor) and NPY (via a Y1-receptor) strongly inhibit, with NPY having a long-lasting effect. Previously it was shown that DA inhibits two (R- and N-type channel) of the four voltage-operated Ca2+ channels in the melanotrope, and that only part of this inhibition is mediated by β/γ-subunits of the Gi protein. We here demonstrate that also the Y1- and GABAB-receptor inhibit only part of the total Ca2+ current (ICa), with fast activation and inactivation kinetics. However, GABAB-mediated inhibition is weaker than the inhibitions induced via Y1- and D2-receptors (â21 versus â27% and â30%, respectively). Using a depolarizing pre-pulse protocol it was demonstrated that GABAB-induced inhibition of ICa most likely depends on Gβ/γ-subunit activation whereas Y1- and D2- induced inhibitions are only partially mediated by Gβ/γ-subunits. No differences were found between the Y1- and D2-induced inhibitions. These results imply that activation of different GiPCR inhibits the ICa through different mechanisms, a phenomenon that may underlie the different potencies of the suprachiasmatic neurotransmitters to inhibit αMSH secretion.
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Endocrinology
Authors
HongYan Zhang, Eric W. Roubos, Bruce G. Jenks, Wim J.J.M. Scheenen,