Effects of acute stress on osmoregulation, feed intake, IGF-1, and cortisol in yearling steelhead trout (Oncorhynchus mykiss) during seawater adaptation

Juvenile steelhead (Oncorhynchus mykiss) acclimated to freshwater (FW) were subjected for 3 h to confinement stress in FW, and subsequently saltwater (SW, 25 ppt) was introduced to all tanks. Fish were sampled immediately after the stress treatment, and 1, 7, and 14 days after introduction of SW. Electrolytes, cortisol, glucose, and lactate showed the typical stress response expected after stress treatment in FW. Fish regained osmotic balance within 24 h. Glucose concentrations were increasing throughout the experiment and lactate levels stayed elevated during the time spent in SW. Insulin-like growth factor-1 (IGF-1) did not show an immediate response to stress but after transfer to SW we detected significantly higher concentrations for control fish at days 1 and 14. The differences in IGF-1 levels between stressed and control fish are not reflected in SW adaptability but positive correlations between IGF-1 and electrolyte levels in control fish may indicate its role for osmoregulation. Confinement stress did not impair feed intake subsequently in SW, but our results suggest that feed intake was suppressed by the change of the media from FW to SW.