| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2814829 | Gene | 2016 | 8 Pages |
•A new stationary phase promoter has been isolated from Gordonia.•The minimal promoter was identified by deletion mapping.•The promoter was found to be growth phase dependent and was most active at stationary phase.•The isolated promoter was found to be six times stronger than Pkan promoter.
Gordonia are gram-positive bacteria belonging to Actinomycetes family with a wide variety of industrial and environmental applications. The genetic toolbox, however, is limited for manipulation of these organisms. In the present study, a new promoter has been isolated from Gordonia sp. IITR 100 and characterized in detail. The promoter was found to be functional in Escherichia coli. The minimal promoter was identified in a 166 bp fragment by deletion mapping. The putative -35 and -10 hexamer showed four and five nucleotide matches respectively with the E. coli consensus sequence. Three direct repeats and an imperfect inverted repeat upstream to -35 were found. The isolated promoter was found to be six times stronger than the Pkan promoter observed by cloning lacZ downstream to each of them in a plasmid in E. coli. The β-galactosidase activity was maximum at stationary phase and found to be ~ 800 MU for Gordonia sp. IITR 100 and E. coli. This is the first report of a stationary phase promoter isolated and characterized from Gordonia.
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