Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2816118 | Gene | 2014 | 8 Pages |
Abstract
Recent studies using the mouse showed an inverse correlation between the Caveolin 1 gene expression and lactation, and this was regulated by prolactin. However, current study using mammary explants from pregnant mice showed that while insulin (I), cortisol (F) and prolactin (P) resulted in maximum induction of the β-casein gene, FP and IFP resulted in the downregulation of Caveolin 1. Additionally, IF, FP and IFP resulted in the downregulation of Caveolin 2. Immunohistochemistry confirmed localisation of Caveolin 1 specific to myoepithelial cells and adipocytes. Comparative studies with the tammar wallaby showed Caveolin 1 and 2 had 70-80% homology with the mouse proteins. However, in contrast to the mouse, Caveolin 1 and 2 genes showed a significantly increased level of expression in the mammary gland during lactation. The regulation of tammar Caveolin 1 and 2 gene expression was examined in mammary explants from pregnant tammars, and no significant difference was observed either in the absence or in the presence of IFP.
Keywords
PBSRibosomal protein S15MicrometreWAPBeta lactoglobulinCaV2μgCAV1CAV3STAT5μMμLGAPDHBLGqPCRBSAcDNATammar wallaby°Cbovine serum albumincomplementary deoxyribonucleic acidRNAribonucleic acidinsulinstandard error of the meandegree celsiusPercentageCarbon dioxideHourLactationMammary glandPhosphate buffered salineSEMMouseMillilitremicrolitreHormonesquantitative polymerase chain reactionWhey acidic proteinProlactincaveolin 3caveolin 1CO2Caveolincortisolglyceraldehyde 3-phosphate dehydrogenase
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Authors
Sanjana Kuruppath, Julie A. Sharp, Christophe Lefevre, Robyn M. Murphy, Kevin R. Nicholas,