| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2816456 | Gene | 2014 | 4 Pages |
•We have identified sex-specific AFLP markers.•We have analyzed the polymorphisms in female and male.•We have analyzed the polymorphisms cluster by Nei's genetic distances.
To investigate the mechanism of sex determination of crabs, amplified fragment-length polymorphism DNA analysis was applied to identify sex-specific markers for Portunus trituberculatus. Eleven pairs of primers were used to amplify DNA isolated from male and female crabs. A total of 481 and 499 fragments were amplified for females and males, respectively, and the ratios of polymorphic fragments were 20.59–65.52% and 27.27–62.22%, respectively. Distribution difference values between female and male groups for 10 polymorphisms were greater than 40%; Cluster analysis revealed that Nei's genetic distances for 57 polymorphisms clustered according to sex. The results indicate that a difference at the DNA level exists between female and male crabs and provide data for further study on sex determination.
