| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2817350 | Gene | 2013 | 6 Pages |
Invasive tephritid fruit flies are a great threat to agriculture worldwide and warrant serious pest control measures. Molecular strategies that promote embryonic lethality in these agricultural pests are limited by the small amount of nucleotide sequence data available for tephritids. To increase the dataset for sequence mining, we generated an EST database by 454 sequencing of the caribfly, Anastrepha suspensa, a model tephritid pest. This database yielded 95,803 assembled sequences with 24% identified as independent transcripts. The percentage of caribfly sequences with hits to the closely related tephritid, Rhagoletis pomonella, transcriptome was higher (28%) than to Drosophila proteins/genes (18%) in NCBI. The database contained genes specifically expressed in embryos, genes involved in the cell death, sex-determination, and RNAi pathways, and transposable elements and microsatellites. This study significantly expands the nucleotide data available for caribflies and will be a valuable resource for gene isolation and genomic studies in tephritid insects.
► Caribfly sequences are more similar to tephritids than to Drosophila. ► Un-annotated transcripts presumably represent tephritid-specific sequences. ► As-hid, As-rpr, DIAP1, peanut, morgue and deterin are represented in the dataset. ► This study will be valuable for genomic studies in non-drosophilids.
