| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2817374 | Gene | 2013 | 9 Pages |
Growth hormone is an essential polypeptide required for normal growth and development of vertebrates. In this report, striped catfish (Pangasianodon hypophthalmus) growth hormone gene and cDNA were isolated by reverse transcriptase-polymerase chain reaction. The striped catfish growth hormone (scGH) encoding gene contains 5 exons and 4 introns. The cDNA sequence of the scGH gene contains a 603 bp open reading frame and encodes for a 200-aa protein consisting of a putative 22-aa signal peptide and the mature 178-aa protein. The recombinant histidine-tagged scGH protein which expressed in Escherichia coli as inclusion bodies was unfolded, refolded and purified to near-homogeneity by Ni2 +–NTA chromatography. Analysis of the secondary structure content by CD spectroscopy showed that the α-helical content of the refolded scGH is 55%. Elucidation of the folding pathway of scGH by fluorescence spectroscopy showed that denaturation transition of scGH is coincident and cooperative, consistent with the two-state denaturation mechanism. The purified scGH was biologically active and exhibited growth-promoting activity in striped catfish, but not tilapia.
► This article reports the genomic and cDNA sequence of striped catfish growth hormone. ► Striped catfish growth hormone (scGH) encoding gene contains 5 exons and 4 introns. ► Folding pathway of scGH follows the two-state denaturation mechanism. ► Recombinant scGH exhibits growth-promoting activity in striped catfish but not tilapia.
