Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2817622 | Gene | 2012 | 4 Pages |
GPx1 is one of the most important enzymes involved in oxidative balance so that, we studied the phenotype and genotype relationship of GPx1 activity and rs 1800668 (C/T) site and also evaluated the changes of GPx1 kinetic parameters in the rs 1800668 homozygotes. One hundred fifty eight subjects were recruited after clinical exams. The rs 1800668 (C/T) genotype distribution was identified using RFLP-PCR method. The hemolysate GPx1 activity was spectrophotometrically measured in a reaction coupled with glutathione reductase (GR). The GPx1 enzyme was purified using gel filtration chromatography with Sephacryl S-300 column and, Kmapp was studied in the rs 1800668 TT and CC homozygotes. The results showed that the GPx1 activity is significantly associated to the rs 1800668 (C/T) genotype distribution (P < 0.05) so that, the GPx1 activity was high among the CC homozygotes (P < 0.03). In addition, Kmapp for TBHP substrate in the TT homozygote (8.48 μM) was higher than the CC homozygote (5.74 μM). We concluded that the C allele within rs 1800668 position is related to the GPx1activity and may be a potential factor involved in development of inflammatory events.
► We studied the role of rs 1800668 site (C/T) on the hemolysate GPx1activity. ► The hemolysate GPx1 activity related directly to the C allele. ► The polymorphic effect was evaluated using kinetic and prediction studies.