| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2817813 | Gene | 2012 | 6 Pages |
Plant microRNAs have a vital role in various abiotic stress responses by regulating gene expression. Heat stress is one of the most severe abiotic stresses, and affects plant growth and development, even leading to death. To identify heat-responsive miRNAs at the genome-wide level in Populus, Solexa sequencing was employed to sequence two libraries from Populus tomentosa, treated and untreated by heat stress. Sequence analysis identified 134 conserved miRNAs belonging to 30 miRNA families, and 16 novel miRNAs belonging to 14 families. Among these miRNAs, 52 miRNAs from 15 families were responsive to heat stress and most of them were down-regulated. qRT-PCR analysis confirmed that the conserved and novel miRNAs were expressed in P. tomentosa, and revealed similar expression trends to the Solexa sequencing results obtained under heat stress. One hundred and nine targets of the novel miRNAs were predicted. This study opens up a new avenue for understanding the regulatory mechanisms of miRNAs involvement in the heat stress response of trees.
► High-throughput sRNA sequencing of P. tomentosa treated/untreated by heat stress. ► Sixteen novel miRNAs and 52 heat-responsive miRNAs were identified. ► Expressions of selected heat-responsive and novel miRNAs were validated by qRT-PCR. ► One hundred and nine targets were predicted for the novel miRNAs. ► The functions of heat-responsive miRNAs and their targets were discussed.
