A novel protein Jpk induces bacterial cell death through reactive oxygen species

Article ID	Journal	Published Year	Pages	File Type
2817828	Gene	2012	5 Pages	PDF

Abstract

âº Jpk induced E. coli cell death upon expression. âº N-term half of Jpk (AA 1-90) was more toxic than C-term in aerobic condition. âº Jpk-induced bacterial cell death was mediated through the ROS. âº DTT, an antioxidant, rescued the bacterial cells expressing Jpk.

Keywords

IPTG DTT GST homeobox HOX c.f.u.colony formation unit ROS Antioxidant sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE isopropyl β-D-thiogalactopyranoside dithiothreitol Bacterial toxicity glutathione S-transferase Reactive oxygen species