Complement C3 gene polymorphism in renal transplantation (an Iranian experience)

The C3 component of complement has different roles in kidney disease and its local production in donor kidney may affect allograft function and rejection after organ transplantation. A single base substitution in c3 gene (rs2230199), defines two common allelic variants with different mobility on gel electrophoresis: S (Slow) and F (Fast). In order to evaluate the effect of this polymorphism on acute renal allograft rejection, one hundred samples of donor and recipients were collected and genotyping was done by PCR-RFLP method. The allelic frequencies were: C3S = 0.791, C3F = 0.209. There was no significant association between recipient's genotype and acute rejection (p value < 0.05). No correlation between donor genotype and acute rejection was also present. Patients were divided into four groups, according to the recipient and donor genotypes: SS recipients and FS or FF donor, SS recipient and donor, FF or FS recipient and SS donor and FS or FF recipient and donor. There was no significant difference in rate of acute rejection between groups. Although the results didn't find any association between C3 complement polymorphisms and acute allograft rejection, there was no acute rejection in FS or FF donors and SS recipient group.

► No association between C3 polymorphisms and acute rejection was observed. ► There was no acute rejection in FS or FF donors and SS recipient group. ► The allelic frequencies were: C3S = 0.791, C3F = 0.209. ► No significant association between donor genotype and rejection episodes was detected.