| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2818135 | Gene | 2012 | 7 Pages |
A novel cathelicidin-like antimicrobial peptide was identified by mining genome of panda. This peptide (cathelicidin-AM) was synthesized. It showed potential antimicrobial activities against wide spectrum of microorganisms including Gram-negative and -positive bacteria, and fungi. It had similar antimicrobial abilities against both standard and clinically isolated drug-resistant strains. Cathelicidin-AM could rapidly exert its antibacterial activities. It just took less than 1 h to kill all Staphylococcus sciuri at the concentration of 2, 4 or 10 times of minimal inhibitory concentration (MIC) while clindamycin took 6 h. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analysis indicated that cathelicidin-AM killed bacteria by directly affecting bacterial cell wall and membrane. Phylogenetic analysis revealed that the panda cathelicidin had the nearest evolution relationship with dog cathelicidin. The current work provides a novel cathelicidin-like peptide with strong antimicrobial abilities.
► A novel cathelicidin antimicrobial peptide was identified by mining panda genome. ► It showed potential antimicrobial activity against wide spectrum of microorganisms. ► It had similar abilities to kill both standard and drug-resistant strains. ► It just took less than 1 h to kill bacteria while clindamycin took 6 h.
