| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2818199 | Gene | 2012 | 5 Pages |
We have developed a novel vector constructed with pDNA, polyethylenimine (PEI), and mucin 1 (MUC1) aptamer for tumor-targeted gene delivery. The MUC1 aptamer and non-specific aptamer were employed to coat the pDNA/PEI complexes electrostatically and stable nanoparticles were formed. The addition of a non-specific aptamer to the pDNA/PEI complex decreased gene expression in the human lung cancer cell line, A549 cells expressing MUC1 regularly. At the same time, the pDNA/PEI/MUC1 aptamer complex showed higher gene expression than pDNA/PEI/non-specific aptamer complex. Furthermore, the pDNA/PEI/MUC1 aptamer complex showed markedly high gene expression in tumor-bearing mice; thus, pDNA/PEI/MUC1 aptamer complexes are useful as a tumor-targeted gene delivery system with high transfection efficiency.
► We constructed a novel gene delivery vector coated with mucin 1 (MUC1) aptamer. ► MUC1 aptamer coated cationic pDNA/polyethylenimine (PEI) complex, electrostatically. ► The complexes showed high gene expressions in the A549 cells with MUC1 expression.
