Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2818681 | Gene | 2010 | 9 Pages |
IL-22 is a novel T and NK cell cytokine that belongs to the IL-10 cytokine family. Here we report the identification of a bovine IL-22 ortholog that is expressed by mitogen activated bovine peripheral blood γ/δ T cells. The full-length bovine IL-22 cDNA contained a 68 bp 5′-untranslated region (UTR), a 570-bp open reading frame, and a 480-bp 3′-UTR. The deduced pre-IL-22 has 190 amino acid residues containing a secretory signal peptide from amino acids 1–33 and several potential N-glycosylation sites. The mature protein is predicted to be a secreted, α-helical molecule. The bovine IL-22 gene is ∼ 7.5 kb in length and is comprised of five introns and six exons, and the first exon is non-coding. Computer analysis and gel shift assay showed that the - 1132 and - 879 region in the 5′ upstream gene sequence contained putative transcription factor binding sites for STATx, Sox-5/9, Sp1, Ik-1, and AREB6. Mutagenesis of STATx and Sox5/9 binding sites decreased promoter functionality by ∼ 50%, suggesting their importance in transcription regulation of IL-22. Expression of IL-22 transcripts induced by various mitogens indicated existence of two regulatory control pathways in γ/δ T cells; IL-2 or PMA treatment induced a slow accumulation of IL-22 mRNA without affecting the maximum induction pathway, whereas ConA treatment rapidly induced a limited amount of IL-22 transcripts. Similar maximal levels of IL-22 transcripts could be induced in γ/δ T cells and α/β T cells. We conclude that bovine γ/δ T cells are important sources of IL-22 and suggest a role for this cytokine in regulating immune responses at mucosal surfaces, including the gut.