Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2819627 | Gene | 2007 | 8 Pages |
Abstract
Immunoscreening of a cDNA expression library, prepared from 7Â days old young shoots of pea (Pisum sativum), identified a novel gene comprising of 2586Â bp open reading frame (ORF) with 381Â bp and 532Â bp 5â² and 3â²untranslated regions (UTRs), respectively. Sequence analysis of this gene, termed as PeIF5B, revealed striking homology to eukaryotic translation initiation factor eIF5B - a sequence homologue of prokaryotic translation initiation factor IF2. Southern blot analyses indicated that PeIF5B exists as a single copy gene in P. sativum genome. Northern blot hybridization revealed the presence of a 7Â kb transcript in pea plant. In vitro translation using rabbit reticulocyte lysate system yielded a protein corresponding to 116Â kDa which was higher than the calculated value of 96Â kDa. Phylogenetic analyses of PeIF5B placed it closer to eIF5B from yeast, human and Drosophila. Pfam domain search analysis pointed to its likely role as a translation initiation factor. The presence of an eIF5B-like factor in a plant system will aid in better understanding of the mechanism of translation initiation in plants.
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Authors
Sheeba Rasheedi, Sudip Ghosh, Madhuri Suragani, Narendra Tuteja, Sudhir K. Sopory, Seyed E. Hasnain, Nasreen Z. Ehtesham,