Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2820135 | Gene | 2006 | 11 Pages |
Abstract
Genes encoding for the two evolutionary highly conserved subunits of a heterotetrameric protein kinase CK2 holoenzyme are present in all examined eukaryotic genomes. Depending on the organism, multiple transcription units encoding for a catalytically active CK2α subunit and/or a regulatory CK2β subunit may exist. The phosphotransferase activity of members of the protein kinase CK2α family is thought to be independent of second messengers but is modulated by interaction with CK2β-like proteins. In the genome of Drosophila melanogaster, one gene encoding for a CK2α subunit and three genes encoding for CK2β-like proteins are present. The X-linked DmCK2β transcription unit encodes for several CK2β protein isoforms due to alternative splicing of its primary transcript. We addressed the question whether CK2β-like proteins are redundant in function. Our in vivo experiments show that variations of the very C-terminal tail of CK2β isoforms encoded by the X-linked DmCK2β transcription unit influence their functional properties. In addition, we find that CK2β-like proteins encoded by the autosomal D. melanogaster genes CK2βtes and CK2βⲠcannot fully substitute for a loss of CK2β isoforms encoded by DmCK2β.
Keywords
SDSCHK2MBUSTEChk1CK2PAGEUASmRNAHEKcDNADNA complementary to RNAmessenger RNAcheckpoint kinase 1checkpoint kinase 2Gene trapupstream activator sequenceVolumedouble-strandedyellowHourStellatesodium dodecyl sulfateWhiteSAPDrosophila melanogasterwild-typehemagglutininpolymerase chain reactionPCRProtein kinase CK2Casein kinase 2human embryonic kidney
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Genetics
Authors
Eike Jauch, Heike Wecklein, Felix Stark, Mandy Jauch, Thomas Raabe,