Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2820447 | Gene | 2006 | 5 Pages |
Abstract
C. elegans small RNAs (<Â 50 nt) were separated by two-dimensional gel electrophoresis (2D-PAGE). cDNAs were prepared from the RNAs extracted from randomly chosen 2D-PAGE spots. Although many cDNA sequences corresponded to parts of known RNAs, twelve novel small RNA candidates were identified: eleven from 2D-PAGE spots of the mixed-stage worm RNA preparation and one from those of the embryonic RNA preparation. These are encoded in the intergenic regions, in the introns of protein-coding genes, in the anti-sense strand of protein-coding sequences and repetitive sequence regions of the genome. None of them showed a characteristic structure of miRNAs, suggesting that they are candidates of other or new classes of RNAs.
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Authors
Yusuke Hokii, Akiyoshi Kubo, Takahiro Ogasawara, Yuhkou Nogi, Akito Taneda, Risa Arai, Akira Muto, Chisato Ushida,