| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2820653 | Genomics | 2013 | 7 Pages |
To investigate the molecular mechanism of silkworm resistance to BmNPV infection, we constructed a near-isogenic line (BC8) with BmNPV resistance using highly resistant (NB) and highly susceptible parental strains (306). We investigated variations in the gene expression in the midguts of BmNPV-infected BC8 and 306 at 12 h pi using the microarray. 92 differentially expressed genes were identified. Real-time qPCR analysis confirmed that 10 genes were significantly up-regulated or down-regulated in the midguts of BC8 and NB compared to 306. To our knowledge, we first defined the role of the amino acid transporter and 26S proteasome in insect antiviral. However, serine protease was not completely consistent with data of reported previously in insect antiviral. The role of the 5 genes (Bm123, Bm122, COP β′, aquaporin, glycoside hydrolases) was also demonstrated in insect antiviral. Our results provided new insights into the molecular mechanism of the Bombyx mori immune response against BmNPV infection.
► 92 differentially expressed genes involved in the resistance to BmNPV were identified. ► qPCR confirmed that 8 or 2 genes were up /down-regulated in NB, BC8 compared to 306. ► We first defined the role of amino acid transporter and 26S proteasome in antiviral. ► Serine protease was not consistent with data of reported previously in antiviral. ► The role of Bm123, Bm122, COP β′, GHs, aquaporin was also demonstrated in antiviral.
