Article ID Journal Published Year Pages File Type
2820932 Genomics 2012 8 Pages PDF
Abstract

DNA methylation changes in repetitive elements (REs) are associated with the regulation of gene transcription, embryonic development, differentiation and carcinogenesis. However, genome-wide analysis of DNA methylation of human REs is lacking. Here, we performed genome-wide methylation analysis of REs in nine repeat types in human embryonic stem cells (H1) and fetal fibroblasts (IMR90), and found that the potential for changes in the DNA methylation of REs was different among the nine repeat types and within different genomic regions. DNA methylation changes in the nine repeat types were related to the GC content and CpG density of the sequence contexts. The differentially methylated REs and targeted genes of different repeat types were associated with gene silencing in the transition from H1 to IMR90 cells. Our results suggest that a quarter of REs are involved in the reprogramming of DNA methylation which may play important epigenetic roles during cellular differentiation.

► Nine repeat types show DNA methylation difference between human H1 and IMR90 cells. ► DNA methylation changes of repeat types related to sequence features and locations. ► Targeted gene expression of differentially methylated REs descends from H1 to IMR90.

Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Genetics
Authors
, , , , , ,