Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2821697 | Genomics | 2007 | 5 Pages |
Abstract
In this paper we describe a method that uses the nearly covalent strength biotin–streptavidin interaction to attach a paramagnetic bead of micrometer size to a DNA molecule of nanometer size, scaling up the spatial size of a query DNA strand by a factor of 1000, making it visible to the human eye. The use of magnetic principles enables rapid binding and washing of detector beads, facilitating a readout of amplified DNA sequences in a few minutes. Here we exemplify the method on mitochondrial DNA variations using an array platform. Visual identification and documentation can be performed with an ordinary mobile phone equipped with a built-in camera.
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Biochemistry, Genetics and Molecular Biology
Genetics
Authors
Patrik L. Ståhl, Jesper Gantelius, Christian Natanaelsson, Afshin Ahmadian, Helene Andersson-Svahn, Joakim Lundeberg,