Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2824330 | Plasmid | 2009 | 4 Pages |
Abstract
The tetracycline-inducible expression vector pALC2073 allowed high level expression of the cloned sasG gene but repression by uninduced cells was leaky. The −10 box of the tetR promoter was mutated to the Bacillus subtitlis consensus, which resulted in complete repression of SasG protein expression. Anhydrotetracycline at 1.28 μg ml−1 gave the same high level of induction that was obtained with pALC2073sasG using 160 ng ml−1 tetracycline, the highest concentration that could be used without inhibiting bacterial growth. This variant of pALC2073 thus offers almost complete repression when uninduced and high levels of expression when induced.
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Authors
Rebecca M. Corrigan, Timothy J. Foster,