| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2828801 | Journal of Structural Biology | 2011 | 13 Pages |
Abstract
In-focus phase-plate imaging is particularly beneficial for cryo-TEM because it offers a substantial overall increase in image contrast, without an electron dose penalty, and it simplifies image interpretation. We show how phase-plate cryo-TEM can be implemented with an appropriate existing TEM, and provide a basic practical introduction to use of thin-film (carbon) phase plates. We point out potential pitfalls of phase-plate operation, and discuss solutions. We provide information on evaluating a particular TEM for its suitability.
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Authors
Michael Marko, ArDean Leith, Chyongere Hsieh, Radostin Danev,