Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2829933 | Molecular and Biochemical Parasitology | 2010 | 5 Pages |
YCF45 belongs to a family of proteins of unknown function usually located in the chloroplast of plants. Its highly conserved homologues were found in the genomes of several Trypanosoma and Leishmania species. HA3-tagging of the YCF45 protein with the start codon as annotated in the GeneDB revealed its cytosolic localization in the cultured procyclic stage of Trypanosoma brucei. However, when a more upstream located start codon was used in another HA3-tagged construct, the resulting protein was targeted to the mitochondrion. We propose that YCF45 was acquired by an ancestral trypanosomatid by horizontal gene transfer and in the absence of a plastid was re-targeted to the mitochondrion.
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