Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2830076 | Molecular and Biochemical Parasitology | 2009 | 4 Pages |
Abstract
Study of the formation of the sexual blood stages of the malaria parasite has been significantly hampered by the absence of a reliable, reproducible assay devoid of operator bias and error. Here we report on the development of an assay utilizing a green fluorescent protein chimera of the early sexual blood stage protein Pfs16 as a marker for commitment to gametocytogenesis. Analysis of parasites via fluorescence activated cell sorting allows for the accurate assessment of gametocyte production well before morphological changes are apparent.
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Authors
Matthew W.A. Dixon, Christopher L. Peatey, Donald L. Gardiner, Katharine R. Trenholme,