Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2830226 | Molecular and Biochemical Parasitology | 2007 | 8 Pages |
Abstract
Protozoan parasites of the genus Leishmania have a digenetic lifecycle, alternating between the promastigote and amastigote stages. The extracellular promastigote resides within a sandfly vector, while the obligate intracellular amastigote stage replicates in the phagolysosome of mammalian host macrophages. Adaptation to and survival within these vastly differently environments is accompanied by differential expression of a subset of genes, which is regulated post-transcriptionally via cis-acting elements in 3â² untranslated region (3â²UTR) or intercistronic sequences. It was reported previously that Leishmania mexicana A600-4 mRNA transcript abundance was eight-fold higher in the amastigotes. In this study, chimeric luciferase:A600-4 3â²UTR reporter constructs were integrated at the A600 chromosome locus to identify regulatory regions of the A600-4 3â²UTR sequence. Evidence is provided for distinct 3â²UTR elements that function to stabilize the A600-4 mRNA transcript in the amastigote stage and to regulate translation efficiency, respectively.
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Authors
Angus Murray, Christine Fu, Golareh Habibi, W. Robert McMaster,