Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2830274 | Molecular and Biochemical Parasitology | 2008 | 5 Pages |
Abstract
Trypanosoma brucei regulates gene expression by post-transcriptional mechanisms, such as mRNA turnover and translation control. This regulation frequently requires specific sequences located in the 3â²-untranslated region. Microarray analysis and Northern blot hybridization showed that the amino acid transporter 11 mRNA is up-regulated in insect stages of the parasite. By RT-PCR and sequencing, the AATP11 polyadenylation site was mapped. We show that this 3â²-UTR causes higher expression of the chloramphenicol acetyltransferase (CAT) reporter gene in procyclic trypanosomes than in bloodstream forms. Results of deletion analysis suggested that multiple elements located between nucleotides 141 and 618 of the 3â²-untranslated region are required for this control.
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Authors
Ana Robles, Christine Clayton,