Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2830483 | Molecular and Biochemical Parasitology | 2006 | 11 Pages |
Abstract
In trypanosomatids all mRNAs undergo trans-splicing, whereas cis-splicing is restricted to a few transcripts. Trans-splicing is mechanistically similar to cis-splicing, however, little is known about the trans-splicing machinery and its underlying mechanism. In this study, we examined the involvement of splicing factors in cis- and trans-splicing by RNA interference (RNAi). Two factors (Prp31 and Prp43) were found to be essential for both pathways, suggesting that splicing factors are shared by these two reactions. We identified a 45S complex carrying pre-mRNA and all the U-snRNAs, including U1 and the SL RNA, suggesting that a single spliceosomal complex may potentially conduct both trans- and cis-splicing.
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Authors
Xue-hai Liang, Qing Liu, Li Liu, Christian Tschudi, Shulamit Michaeli,