Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2830850 | Molecular Immunology | 2014 | 9 Pages |
•ICOS costimulation activates mTOR pathway in activated T cells through PI3K.•ICOS-PI3K-mTOR signaling enhances polysome formation on IL-4 mRNA.•PI3K-mediated ICOS costimulation may facilitate delivery of IL-4 to cognate B cells.
The inducible costimulator (ICOS) is highly expressed in follicular helper T (Tfh) cells, a subset of CD4 T cells that migrate into the B cell zone and facilitate germinal center reactions. Although ICOS is known to play a critical role in forming the Tfh cell population during immune responses, its contribution to the effector functions of Tfh cells remains unclear. Using activated mouse splenic CD4 T cells we demonstrate that ICOS assists TCR-mediated signal transduction by potentiating the PI3K-AKT-mTOR signaling cascade that leads to hyper-phosphorylation of p70S6K and 4E-BP1, events that are known to augment cap-dependent mRNA translation. Consequently, ICOS costimulation promotes the formation of polysomes on IL-4 mRNA in a PI3K-dependent manner. Furthermore, we show that the supply of IL-4 becomes a limiting factor for T-dependent B cell activation during in vitro co-culture when the ICOS-PI3K signaling axis is disrupted in T cells. This ICOS costimulation-dependent translational control may ensure targeted delivery of IL-4 to cognate B cells during T–B collaborations in the germinal center.