| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2830941 | Molecular Immunology | 2012 | 8 Pages |
The mechanisms of each subset of immune cells contributing to the pathogenesis of viral hepatitis remain incompletely understood. In this study, we examined the role of liver CD4− CD8− (double negative, DN) T cells during murine hepatitis virus strain 3 (MHV-3)-induced hepatitis in C3H/HeJ mice. We demonstrate that predominant population of DN T cells in the liver of healthy or MHV-3-infected mice express TCRγδ+. The proportion of TCRγδ+ DN T cells in liver CD3+ T cells was markedly increased after MHV-3 infection. Adoptive transfer of TCRγδ+ DN T cells led to dramatically decreased survival in MHV-3-infected mice, accompanied by deteriorated histopathology and elevated ALT and AST levels. It was found that these cells were hyperactivated after MHV-3 infection with a production of TNF-α, IFN-γ, IL-2 and IL-17A. Highly activated liver TCRγδ+ DN T cells were cytotoxic to MHV-3-infected hepatocytes in vitro and this effect did not require cell-cell contact. Moreover, the cytotoxic effect of liver TCRγδ+ DN T cells against hepatocytes involves TNF-α pathway, but not IL-17A or IFN-γ. These results indicate that liver TCRγδ+ DN T cells play a critical role in the liver injury in MHV-3-induced hepatitis, via a TNF-α dependent pathway.
► Predominant population of DN T cells in the liver of healthy or MHV-3-infected mice express TCRγδ+. ► Liver TCRγδ+ DN T cells increased and highly activated after MHV-3 infection. ► Adoptive transfer of TCRγδ+ DN T cells led to dramatically decreased survival in MHV-3-infected mice. ► Liver TCRγδ+ DN T cells produced TNF-α, IFN-γ, IL-2 and IL-17A post-MHV-3 infection. ► The cytotoxicity of liver TCRγδ+ DN T cells against MHV-3-infected hepatocytes was TNF-α-mediated.
