| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2831239 | Molecular Immunology | 2011 | 6 Pages |
Abstract
⺠Consistent assessment of ADCC activity of therapeutic antibodies is technically challenging due to donor-dependent variation of primary effector cells. ⺠Here we report on the development of a technically convenient quantitative assay based on genetically engineered effector cell lines. ⺠The assay system is highly reproducible and exhibits the hallmarks of traditional ADCC assays based on PBMCs. ⺠We demonstrate a high sensitivity of the assay to even small changes in antibody fucosylation.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Molecular Biology
Authors
Alfred Schnueriger, Roger Grau, Peter Sondermann, Thomas Schreitmueller, Stefan Marti, Marcel Zocher,