Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2831564 | Molecular Immunology | 2009 | 4 Pages |
Abstract
Purification of lymphocytes, particularly T cells, is commonly performed using nylon wool. This enrichment method selectively retains B cells and some myeloid cells allowing a significantly more pure T cell population to flow through a nylon wool column. T cells purified in this fashion are assumed to be unaltered and functionally naïve, however some studies have suggested aberrant in vitro T cell responses after nylon wool treatment. We found that nylon wool purification significantly altered T cell proliferation, expression of activation markers and production of cytokines. Our results suggest that nylon wool treatment modifies T cell activation responses and that caution should be used when choosing this purification method.
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Authors
Jillian E. Wohler, Scott R. Barnum,