Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2833043 | Molecular Immunology | 2006 | 9 Pages |
Abstract
Synthetic oligodeoxynucleotides (CpG-ODNs) and bacterial DNA containing unmethylated CpG dinucleotides in the context of particular base sequences (CpG motifs) are known to inhibit anti-IgM-induced growth arrest and apoptosis of WHEI 231 B lymphocytes, and spontaneous apoptosis of mature spleen B cells in a sequence-specific fashion of the CpG-ODN. Here we report that CpG-ODN protects from the cell death induced by γ-irradiation of primary mouse spleen cells as well as mouse RAW 264.7 macrophage cells and human RPMI 8226 B cells. Experimental results showed that CpG-ODN promotes growth of the cells, and protects the cells from γ-irradiation-induced cell death accompanying Bcl-xS/L and Bcl-2 upregulation. Furthermore, survival of macrophages was enhanced when splenocytes were pretreated with CpG-ODN. Our results suggest the potential application of CpG-ODNs for more efficient cancer radiotherapy by enhancing survival of normal immune cells after radiation damage.
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Authors
Wern-Joo Sohn, Keun-Wook Lee, Soo Young Choi, Eunkyung Chung, Younghee Lee, Tae Yoon Kim, Suk Kyeong Lee, Yong-Kyoung Choe, Jeung-Hoon Lee, Doo-Sik Kim, Hyung-Joo Kwon,