Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2833274 | Molecular Immunology | 2007 | 10 Pages |
Prolactin (PRL) and growth hormone (GH) (somatotropin) have been known to possess immunomodulatory properties. In the present studies we have investigated the production of nitric oxide (NO) and TNF-α by murine peritoneal macrophages in vitro on treatment with PRL and GH and the signal transduction mechanism involved. It is observed that significantly enhanced production of NO is induced in macrophages on treatment with PRL and GH. It is further observed that protein tyrosine kinases, MAP kinases and Ca++ channeling are involved in NO production by macrophages on in vitro treatment with PRL and GH. GH and PRL induced nitric oxide did not have any effect on the expression and production of TNF-α. PRL or GH induced TNF-α production by murine macrophages was insensitive in the presence of competitive inhibitor of NOS, l-NMMA. Similarly, there is no autocrine or paracrine effect of TNF-α on GH or PRL induced NO production and iNOS expression.