Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2833346 | Molecular Immunology | 2007 | 8 Pages |
Abstract
Crosslinking of FcÉ receptor on mast cells induces IL-3 gene expression with the concentration dependent of intracellular calcium, but its regulatory mechanism remains unclear. Here, we found that phorbol 12-myristate 13-acetate (PMA) alone did not induce IL-3 gene expression, but potentiated A23187-induced IL-3 gene expression. Interestingly, the A23187-induced IL-3 promoter activity was suppressed by PMA, but it was enhanced when IL-3 promoter contained enhancer region, a DH site. While IL-3 mRNA expression was increased by A23187 and PMA in a dose-dependent manner, the promoter activity appeared all or none in all doses of A23187 and PMA. IL-3 promoter region between â293 and â150Â bp was responsible for A23187-induced gene expression and PMA- or cyclosporin A (CsA)-mediated suppression. Taken together, IL-3 gene expression was primarily regulated at the transcriptional level, which was differentially controlled by a restricted promoter and enhancer region.
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Authors
Chang-Bo Ko, Bok-Soo Lee, Seok-Ho Cha, Donggeun Sul, Sang-Gi Paik, Hyung-Sik Kang,