| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2914113 | European Journal of Vascular and Endovascular Surgery | 2010 | 7 Pages |
ObjectiveThis study aimed to compare arteriogenesis after femoral artery occlusion as influenced by exercise or arteriovenous shunt and follow changes in collateral transient receptor potential cation channel, subfamily V, member 4 (Trpv4).DesignA prospective, controlled study wherein rats were subjected to femoral artery ligation (FAL), or FAL + arteriovenous shunt. Collateral Trpv4 was determined 0.5 and 6 h post exercise.MethodsRats were subjected to exercise for 15 min, twice daily. The number and diameter of collaterals were assessed after 7 days. Collateral Trpv4 expression was quantified by reverse transcription-polymerase chain reaction.ResultsCollateral number and diameter per limb were significantly higher in the shunt group (number: 16.0 ± 2.4 and diameter: 216.0 ± 34 μm) compared to the ligature (number: 9.4 ± 2 and diameter: 144 ± 21 μm) and exercise groups (number: 9.9 ± 2.5 and diameter: 151 ± 15 μm). Trpv4 expression in collaterals harvested 0.5 h post exercise was not significantly different from expression in shunted rats. It was significantly lower in collaterals harvested 6 h post exercise (comparable to that in ligated rats).ConclusionCollateral formation was greater in the shunt group than in the exercise group. Exercise-induced Trpv4 up-regulation, not significantly different from that achieved with shunt, returned to control values when evaluated 6 h post exercise. More frequent exercise to chronically increase fluid shear stress, as with a shunt model, may be required for sufficient arteriogenesis to compensate for peripheral occlusion.
