Autologous human plasma in stem cell culture and cryopreservation in the creation of a tissue-engineered vascular graft

Autologous adipose-derived stem cells have been shown to be effective endothelial cell substitutes in the creation of tissue-engineered vascular grafts in previous in vitro and in vivo animal studies. This study evaluates two aspects of translating this work into human use, namely, use of autologous plasma instead of fetal bovine serum in cell culture (improving safety) and cryopreservation (improving accessibility).