Ex Vivo Lung Perfusion

The number of patients listed for lung transplantation largely exceeds the number of available transplantable organs because of both a shortage of organ donors and a low utilization rate of lungs from those donors. A novel strategy of donor lung management – Ex vivo Lung Perfusion (EVLP) - that keeps the organ at physiological protective conditions have shown a great promise to increase lung utilization by reevaluating, treating, and repairing donor lungs prior to transplantation. Clinical trials using EVLP has shown the method to be safe and allow for reassessment and improvement in function from high risk donor lungs from both brain death and cardiac death donors prior transplantation. Pre-clinical studies have also shown a great potential of EVLP as a platform for the delivery of novel therapies to repair injured organs ex vivo and thus further improve lung transplantation outcomes. Herein we describe detailed steps for a successful EVLP procedure.