Effects of pretreatments of anaerobic sludge and culture conditions on hydrogen productivity in dark anaerobic fermentation

Effects of various pretreatments of the anaerobic sludge on hydrogen production were investigated for enhancing hydrogen production via dark anaerobic fermentation of organic substrates. The pretreatment methods included heat treatment, acid or base treatment, acid/heat treatment and base/heat treatment. After the pretreatment of the anaerobic sludge, productivity of H2 by the pretreated sludge were evaluated when the culture were grown on glucose-based medium at ambient conditions. The detailed experimental investigation showed highest production of H2 by the heat-treated culture followed by the heat and acid-treated culture and the heat and base-treated culture.