Article ID Journal Published Year Pages File Type
301179 Renewable Energy 2011 5 Pages PDF
Abstract

The expression of the multi-functional cellulase gene mfc in Coprinopsis cinerea is modulated using glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter fragments from Agaricus bisporus, Flammulina velutipes, and Lentinula edodes. In submerged fermentation, with banana peels as the growth substrate, highest activities of endo-β-1,4-glucanase (0.48 U/ml), total cellulase (0.26 U/ml) and endo-β-1,4-xylanase (38.10 U/ml) were noted in the culture liquid Cabm44, which uses a 275 bp gpd fragment from A. bisporus. The lignocellulolytic enzymatic activities were increased nearly twofold in comparison with the wild-type strain.

► The expression of the multi-functional cellulase gene in Coprinopsis cinerea. ► Banana peels can highly induce the production of multi-functional cellulase. ► The enzymatic activities were increased 2-fold using A. bisporus gpd promoter.

Related Topics
Physical Sciences and Engineering Energy Renewable Energy, Sustainability and the Environment
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