| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3027689 | Thrombosis Research | 2010 | 4 Pages |
Platelets are highly reactive components of the circulatory system. The cytoskeleton of a platelet is an important structure for platelet aggregation as stimulated by several agonists. An anticancer agent, taxol, has been suggested to exert platelet anti-aggregating activity by stabilizing microtubules during the aggregation process. An activity-guided fractionation was performed with a methanol extract of the leaves and twigs of Taxus cuspidata to isolate taxanes with platelet anti-aggregating effects. Compounds 1 to 7 – taxinine (1), taxinine A (2), taxinine B (3), 2-deacetoxytaxinine B (4), taxacin (5), taxchinin B (6), and taxol (7) – were obtained as the antiplatelet components of this plant. These taxane compounds present the possibility of securing new antiplatelet compounds which differ from currently available antiplatelet agents in chemical structure and possibly in mechanisms of action. All compounds showed stronger inhibitory effects than acetylsalicylic acid (ASA) on platelet aggregation induced by arachidonic acid (AA) (IC50: 14.4, 64.5, 35.5, 16.0, 21.9, 28.6 and 48.2 versus 63.0 μM) or U46619 (IC50: 34.8, 24.9, 36.2, 35.0, 46.9, 71.9 and 68.7 versus 340 μM). Compounds 1, 3, 4 and 5, with a cinnamoyl group at the C5 position, showed strong inhibitory effects against AA-induced aggregation compared to compound 2 (with an -OH group at C5) or compounds with an oxetane ring at C4,5, such as compounds 6 and 7. All of the seven compounds were 5–13-fold more strongly inhibitory than ASA against U46619-induced aggregation.
