Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3029188 | Thrombosis Research | 2010 | 6 Pages |
Abstract
Calibrated automated thrombin generation assay was adapted to measure thrombin generation in platelet rich plasma from mice. Vena cava phlebotomy appeared the best technique for blood sampling. The concentration-effect curves of tissue factor and platelet count have been determined. Corn trypsin inhibitor 2 μM inhibits contact activation effectively. Recombinant human thrombomodulin does not inhibit thrombin generation in mouse plasma but activated protein C (20 nM) does. Thrombin generation was dose dependently diminished by low molecular weight heparin and increased by high concentrations of exogenous factor VIII i.e. the assay can detect both hypo- and hypercoagulability.
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Authors
Yesim Dargaud, Henri M.H. Spronk, Peter Leenders, H. Coenraad Hemker, Hugo Ten Cate,