Immediate early gene expression invoked by electrical intracochlear stimulation in some but not all types of neurons in the rat auditory brainstem

Specific patterns of sensory activity may induce plastic remodeling of neurons and the communication network they form in the adult mammalian brain. Among the indicators for the initiation of neuronal remodeling is the expression of immediate early genes (IEGs). The IEGs c-fos and egr-1 encode transcription factors. Following spectrally and temporally precisely defined unilateral electrical intracochlear stimulation (EIS) that corresponded in strength to physiological acoustic stimuli and lasted for 2 h under anesthesia, we characterized those neuronal cell types in ventral (VCN) and dorsal cochlear nucleus (DCN), lateral superior olive (LSO) and central nucleus of the inferior colliculus (CIC) of the rat brain that expressed IEGs. We found that EIS affected only specific types of neurons. Whereas sub-populations of glutamatergic and glycinergic cells responded in all four regions, GABAergic neurons failed to do so except in DCN. Combining immunocytochemistry with axonal tracing, neurons participating in major ascending pathways, commissural cells of VCN and certain types of neurons of the descending auditory system were seen to respond to EIS with IEG expression. By contrast, principal LSO cells projecting to the contralateral CIC as well as collicular efferents of the DCN did not. In total, less than 50% of the identified neurons turned up expression of the IEGs studied. The pattern of IEG expression caused by unilateral EIS led us to suggest that dominant sensory activity may quickly initiate a facilitation of central pathways serving the active ear at the expense of those serving the unstimulated ear.