| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3097996 | Chinese Herbal Medicines | 2012 | 8 Pages |
ObjectiveTo investigate the effect of n-butanol extract from Potentilla anserina (NP) intervention on hypoxia-induced Ca2+ overload and SERCA2 expression of rat cardiomyocytes.MethodsPrimary cultured myocardial cell from SD neonatal rat (1–3 d) was used in the establishment of hypoxia model. After hypoxia for 3 h, the Ca2+ concentration of myocardial cells was measured with fura-2/AM fluorescent probe, and the biochemical indicator intracellular Ca2+-ATPase was examined and the mRNA and its protective protein levels of the sarcoplasmic reticulum (SR) Ca2+-ATPases (SERCA2) were assayed with RT-PCR, Western-blotting, and immune-cytochemical staining in each group.ResultsThe results showed that NP decreased Ca2+ concentration, increased the activity of Ca2+-ATPase, and improved the mRNA and protein expression of SERCA2 in hypoxia-injured myocardial cells as compared with the model group.ConclusionThese results indicate that NP could attenuate the Ca2+ overload. The mechanism might be explained as that NP could elevate the SERCA2 level, increase the activity of myocardium in rats, and further enhance the capacity of SR Ca2+ re-uptake.
