Study on Targeting and in vitro Anti-oxidation of Baicalin Solid Lipid Nanoparticles

ObjectiveTo prepare liver-targeted baicalin solid lipid nanoparticles (BSLNs) and to study their in vitro anti-oxidative activity.MethodsBSLNs were prepared by emulsification ultrasonic dispersion method and characterized by transmission electron microscopy and laser particle size distribution; The tissue in vivo distribution was detected by pharmacokinetics; In vitro anti-superoxide dismutase (SOD) activity and reduction capacity of BSLNs were determined; The ability of removing hydroxyl radical was determined by phenanthroline-Fe2+ oxidation.ResultsThe best prescription was baicalin-soybean lecithin-glyceryl monostearate-poloxamer 188 (1:5:15:30); The encapsulation efficiency and drug loading were 84.7% and 5.65%, respectively, mean size of particles was (68.6 8) nm, Zeta potential was −22.13 mV; The in vitro anti-oxidant results showed that BSLNs had a significant inhibitory effect on SOD and a strong reducing capacity as well as a removing hydroxide radical ability. The targeting rate of BSLNs was 6.931 for liver.ConclusionThe results demonstrate that BSLNs could enhance the liver targeting ability and in vitro anti-oxidative activity significantly.