Article ID Journal Published Year Pages File Type
3098107 Chinese Herbal Medicines 2012 7 Pages PDF
Abstract

ObjectiveTo establish an HPLC method for the determination of seven flavonoids from the aerial part of Artemisia frigida.MethodsHypersil ODS-2 (300 mm × 4.6 mm, 5 μm) column was used, with acetonitril-0.2% phosphoric acid (gradient elution) as a mobile phase, and the detection wavelength was at 283 nm with flow rate at 1 mL/min.ResultsAll calibration curves showed good linear regression (r > 0.9990) within the tested range. All average recovery was more than 98.00% and RSD was less than 3.0% (n = 6).ConclusionThe method is steady and with good repeatability, and could be used to determine the content of flavonoids in A. frigida from different areas.

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