Article ID Journal Published Year Pages File Type
3126125 British Journal of Oral and Maxillofacial Surgery 2006 8 Pages PDF
Abstract

Thrombospondin-1 (TSP-1) is an extracellular matrix glycoprotein implicated in the regulation of angiogenesis and tumour development. Our objectives were to ascertain the quantity and quality of RNA extracted from archival, formalin-fixed, paraffin embedded, oral tissues and their application in measuring the concentrations of TSP-1 mRNA in these tissues. We compared three techniques of isolation of RNA as well as related experimental variables. TSP-1 mRNA was measured in specimens of normal, dysplastic, and malignant oral tissues by real-time reverse transcriptase polymerase chain reaction (RT-PCR). RNA suitable for analysis by real-time RT-PCR was obtained by the three techniques tested, although the yield varied depending on the protocol used (range 0.2–3.6 μg/mm3). The mean (S.D.) concentrations of TSP-1 mRNA relative to 18S were 21.1 (7.2) in normal oral tissues (n = 9), 11.0 (8.2) in dysplastic tissue (n = 8) and 7.3 (5.3) in carcinomatous tissue (n = 17). The difference between normal and carcinomatous specimens was significant (p = 0.01). This reduction in expression of TSP-1 mRNA from normal to dysplasia to carcinoma may favour the angiogenic drive that accompanies the development of oral tumours.

Related Topics
Health Sciences Medicine and Dentistry Dentistry, Oral Surgery and Medicine
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